Topics to be covered:
- Developability and stability studies of biologics
- Characterization
- Protein aggregation
- Physical and chemical stability screening
- Developing predictive and correlative tools and application of novel tools
- Workflows from early- and late-stage development
Moderator: Charles Heffern, Product Manager, NanoTemper
Talk 1: Nano Differential Scanning Fluorimetry and Its Applications
Jie Wen, Senior Scientist, Higher Order Structure, Biological Relevance and Characterization, Attribute Sciences Amgen, Thousand Oaks
nanoDSF has emerged as a powerful tool for thermal stability analysis of therapeutic proteins. Compared to the current recognized industry-standard DSC, the nanoDSF method enables thermal stability analysis over a much wider concentration range, consumes
considerably less materials, and provides significantly higher throughput. In this presentation, the advantages of the nanoDSF method for thermal stability analysis are discussed. The examples of the nanoDSF applications, such as developability and
formulation studies as well as comparability studies of therapeutic proteins, are presented.
Talk 2: Get Clarity at The Critical Junctures In Your Biologic Development With Prometheus (15 minutes)
Charles Heffern, Product Manager, NanoTemper
Stability of biopharmaceuticals is a complex mixture of parameters and plays a crucial role through the entire product lifecycle, from discovery through production and manufacturing. Making the critical decisions that determine the success of a biopharmaceutical
requires clear and precise results. Here, we discuss how Prometheus empowers experienced organizations to improve their biologic discovery and development by using high-quality data to make better decisions.
Q&A (15 minutes)
Charles Heffern, Product Manager, NanoTemper
Jie Wen, Senior Scientist, Higher Order Structure, Biological Relevance and Characterization, Attribute Sciences Amgen, Thousand Oaks
Jie Wen
Senior Scientist, Higher Order Structure, Biological Relevance and Characterization, Attribute Sciences
Amgen, Thousand Oaks
Jie Wen is currently a senior scientist at Amgen. He is working in the Higher Order Structure (HOS) group. Since 1992, he has been working in biophysical characterization and HOS at Amgen. His work has resulted in more than 30 publications. His research interests include HOS, protein stability, and biophysical characterization of proteins, protein-protein interactions, and protein-small molecule interactions using nanoDSF, DSC, ITC, and other biophysical techniques.
Charles Heffern
Product Manager, NanoTemper
Charles Heffern received his Bachelors of Science in Biochemistry at the University of Southern California in Los Angeles. He went on to pursue his graduate work at the University of Chicago within the Physical Chemistry department. Charles has been working as Application Scientist for NanoTemper Technologies since February 2015.